TY  - JOUR
A1  - Cianciosi, Danila
A1  - Ansary, Johura
A1  - Forbes-Hernandez, Tamara Y.
A1  - Regolo, Lucia
A1  - Quinzi, Denise
A1  - Gracia Villar, Santos
A1  - Garcia Villena, Eduardo
A1  - Tutusaus Pifarre, Kilian
A1  - Alvarez-Suarez, José M.
A1  - Battino, Maurizio
A1  - Giampieri, Francesca
IS  - 9
AV  - public
JF  - Molecules
N2  - Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38?). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture.
ID  - uninimx105
TI  - The Molecular Basis of Different Approaches for the Study of Cancer Stem Cells and the Advantages and Disadvantages of a Three-Dimensional Culture
VL  - 26
Y1  - 2021/04//
UR  - http://doi.org/10.3390/molecules26092615
SN  - 1420-3049
KW  - Cancer stem cells
KW  -  Identification
KW  -  Isolation
KW  -  Enrichment
KW  -  Methodology
KW  -  Three-dimensional culture
KW  -  Surface molecular markers
KW  -  Functional assays.
ER  -