eprintid: 105 rev_number: 17 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/05 datestamp: 2021-11-08 15:59:17 lastmod: 2023-07-10 23:30:09 status_changed: 2021-11-08 15:59:17 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Cianciosi, Danila creators_name: Ansary, Johura creators_name: Forbes-Hernandez, Tamara Y. creators_name: Regolo, Lucia creators_name: Quinzi, Denise creators_name: Gracia Villar, Santos creators_name: Garcia Villena, Eduardo creators_name: Tutusaus Pifarre, Kilian creators_name: Alvarez-Suarez, José M. creators_name: Battino, Maurizio creators_name: Giampieri, Francesca creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: santos.gracia@uneatlantico.es creators_id: eduardo.garcia@uneatlantico.es creators_id: kilian.tutusaus@uneatlantico.es creators_id: creators_id: creators_id: title: The Molecular Basis of Different Approaches for the Study of Cancer Stem Cells and the Advantages and Disadvantages of a Three-Dimensional Culture ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica divisions: uninimx_produccion_cientifica full_text_status: public keywords: Cancer stem cells, Identification, Isolation, Enrichment, Methodology, Three-dimensional culture, Surface molecular markers, Functional assays. abstract: Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38−). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture. date: 2021-04 date_type: published publication: Molecules volume: 26 number: 9 pagerange: 2615 pages: 0 id_number: doi:10.3390/molecules26092615 refereed: TRUE issn: 1420-3049 official_url: http://doi.org/10.3390/molecules26092615 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 access: open language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Universidad Internacional Iberoamericana México > Investigación > Producción Científica Abierto Inglés Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38−). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture. metadata Cianciosi, Danila; Ansary, Johura; Forbes-Hernandez, Tamara Y.; Regolo, Lucia; Quinzi, Denise; Gracia Villar, Santos; Garcia Villena, Eduardo; Tutusaus Pifarre, Kilian; Alvarez-Suarez, José M.; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, santos.gracia@uneatlantico.es, eduardo.garcia@uneatlantico.es, kilian.tutusaus@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2021) The Molecular Basis of Different Approaches for the Study of Cancer Stem Cells and the Advantages and Disadvantages of a Three-Dimensional Culture. Molecules, 26 (9). p. 2615. ISSN 1420-3049 document_url: http://repositorio.unini.edu.mx/id/eprint/105/1/molecules-26-02615-v4.pdf