eprintid: 2918 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/00/29/18 datestamp: 2022-07-21 23:30:03 lastmod: 2022-07-21 23:30:04 status_changed: 2022-07-21 23:30:03 type: article metadata_visibility: show creators_name: González-Peña, Sandra M. creators_name: Calvo-Anguiano, Geovana creators_name: Martínez-de-Villarreal, Laura E. creators_name: Ancer-Rodríguez, Patricia R. creators_name: Lugo-Trampe, José J. creators_name: Saldivar-Rodríguez, Donato creators_name: Hernández-Almaguer, María D. creators_name: Calzada-Dávila, Melissa creators_name: Guerrero-Orjuela, Ligia S. creators_name: Campos-Acevedo, Luis D. title: Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study ispublished: pub subjects: uneat_sn divisions: uninimx_produccion_cientifica full_text_status: public keywords: folic acid intake; ventricular septal defects; congenital heart disease; methylation status; AXIN1; MTHFR; TBX1; TBX20 abstract: Background: DNA methylation is the best epigenetic mechanism for explaining the interactions between nutrients and genes involved in intrauterine growth and development programming. A possible contributor of methylation abnormalities to congenital heart disease is the folate methylation regulatory pathway; however, the mechanisms and methylation patterns of VSD-associated genes are not fully understood. Objective: To determine if maternal dietary intake of folic acid (FA) is related to the methylation status (MS) of VSD-associated genes (AXIN1, MTHFR, TBX1, and TBX20). Methods: Prospective case–control study; 48 mothers and their children were evaluated. The mothers’ dietary variables were collected through a food frequency questionnaire focusing on FA and the consumption of supplements with FA. The MS of promoters of genes was determined in the children. Results: The intake of FA supplements was significantly higher in the control mothers. In terms of maternal folic acid consumption, significant differences were found in the first trimester of pregnancy. Significant differences were observed in the MS of MTHFR and AXIN1 genes in VSD and control children. A correlation between maternal FA supplementation and MS of AXIN1 and TBX20 genes was found in control and VSD children, respectively. Conclusions: A lower MS of AXIN1 genes and a higher MS of TBX20 genes is associated with FA maternal supplementation. date: 2021 publication: Nutrients volume: 13 number: 6 pagerange: 2071 id_number: doi:10.3390/nu13062071 refereed: TRUE issn: 2072-6643 official_url: http://doi.org/10.3390/nu13062071 access: open language: en citation: Artículo Materias > Alimentación Universidad Internacional Iberoamericana México > Investigación > Producción Científica Abierto Inglés Background: DNA methylation is the best epigenetic mechanism for explaining the interactions between nutrients and genes involved in intrauterine growth and development programming. A possible contributor of methylation abnormalities to congenital heart disease is the folate methylation regulatory pathway; however, the mechanisms and methylation patterns of VSD-associated genes are not fully understood. Objective: To determine if maternal dietary intake of folic acid (FA) is related to the methylation status (MS) of VSD-associated genes (AXIN1, MTHFR, TBX1, and TBX20). Methods: Prospective case–control study; 48 mothers and their children were evaluated. The mothers’ dietary variables were collected through a food frequency questionnaire focusing on FA and the consumption of supplements with FA. The MS of promoters of genes was determined in the children. Results: The intake of FA supplements was significantly higher in the control mothers. In terms of maternal folic acid consumption, significant differences were found in the first trimester of pregnancy. Significant differences were observed in the MS of MTHFR and AXIN1 genes in VSD and control children. A correlation between maternal FA supplementation and MS of AXIN1 and TBX20 genes was found in control and VSD children, respectively. Conclusions: A lower MS of AXIN1 genes and a higher MS of TBX20 genes is associated with FA maternal supplementation. metadata González-Peña, Sandra M.; Calvo-Anguiano, Geovana; Martínez-de-Villarreal, Laura E.; Ancer-Rodríguez, Patricia R.; Lugo-Trampe, José J.; Saldivar-Rodríguez, Donato; Hernández-Almaguer, María D.; Calzada-Dávila, Melissa; Guerrero-Orjuela, Ligia S. y Campos-Acevedo, Luis D. mail SIN ESPECIFICAR (2021) Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study. Nutrients, 13 (6). p. 2071. ISSN 2072-6643 document_url: http://repositorio.unini.edu.mx/id/eprint/2918/1/nutrients-13-02071.pdf