TY - JOUR Y1 - 2014/// N2 - Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and ?-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging. SP - 7798 A1 - Giampieri, Francesca A1 - Alvarez-Suarez, José A1 - Mazzoni, Luca A1 - Forbes-Hernandez, Tamara Y. A1 - Gasparrini, Massimiliano A1 - Gonzàlez-Paramàs, Ana A1 - Santos-Buelga, Celestino A1 - Quiles, José A1 - Bompadre, Stefano A1 - Mezzetti, Bruno A1 - Battino, Maurizio UR - http://doi.org/10.3390/molecules19067798 AV - public EP - 7816 KW - strawberry; anthocyanin; ROS; lipid peroxidation; DNA damage; fibroblasts JF - Molecules ID - uninimx612 TI - Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality IS - 6 SN - 1420-3049 VL - 19 ER -