TY  - JOUR
SN  - 2079-6382
ID  - uninimx6352
UR  - http://doi.org/10.3390/antibiotics12030511
TI  - Cytotoxicity and Antimicrobial Resistance of Aeromonas Strains Isolated from Fresh Produce and Irrigation Water
N2  - The genus Aeromonas has received constant attention in different areas, from aquaculture and veterinary medicine to food safety, where more and more frequent isolates are occurring with increased resistance to antibiotics. The present paper studied the interaction of Aeromonas strains isolated from fresh produce and water with different eukaryotic cell types with the aim of better understanding the cytotoxic capacity of these strains. To study host-cell pathogen interactions in Aeromonas, we used HT-29, Vero, J774A.1, and primary mouse embryonic fibroblasts. These interactions were analyzed by confocal microscopy to determine the cytotoxicity of the strains. We also used Galleria mellonella larvae to test their pathogenicity in this experimental model. Our results demonstrated that two strains showed high cytotoxicity in epithelial cells, fibroblasts, and macrophages. Furthermore, these strains showed high virulence using the G. mellonella model. All strains used in this paper generally showed low levels of resistance to the different families of the antibiotics being tested. These results indicated that some strains of Aeromonas present in vegetables and water pose a potential health hazard, displaying very high in vitro and in vivo virulence. This pathogenic potential, and some recent concerning findings on antimicrobial resistance in Aeromonas, encourage further efforts in examining the precise significance of Aeromonas strains isolated from foods for human consumption.
A1  - Pintor-Cora, Alberto
A1  - Tapia Martínez, Olga
A1  - Elexpuru Zabaleta, Maria
A1  - Ruiz de Alegría, Carlos
A1  - Rodríguez-Calleja, Jose M.
A1  - Santos, Jesús A.
A1  - Ramos Vivas, Jose
AV  - public
JF  - Antibiotics
VL  - 12
KW  - Aeromonas; virulence; cytotoxicity; antimicrobial resistance; host?pathogen interactions
Y1  - 2023///
IS  - 3
ER  -