Documentos donde el Autor es "Quiles, José L."

2025

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional do Cuanza > Investigación > Producción Científica
Universidad de La Romana > Investigación > Producción Científica Cerrado Inglés Understanding how dietary compounds affect human health is challenged by their molecular complexity and cell-type–specific effects. Conventional multi-cell type (bulk) analyses obscure cellular heterogeneity, while animal and standard in vitro models often fail to replicate human physiology. Single-cell omics technologies—such as single-cell RNA sequencing, as well as single-cell–resolved proteomic and metabolomic approaches—enable high-resolution investigation of nutrient–cell interactions and reveal mechanisms at a single-cell resolution. When combined with advanced human-derived in vitro systems like organoids and organ-on-chip platforms, they support mechanistic studies in physiologically relevant contexts. This review outlines emerging applications of single-cell omics in nutrition research, emphasizing their potential to uncover cell-specific dietary responses, identify nutrient-sensitive pathways, and capture interindividual variability. It also discusses key challenges—including technical limitations, model selection, and institutional biases—and identifies strategic directions to facilitate broader adoption in the field. Collectively, single-cell omics offer a transformative framework to advance human-centric nutrition research. metadata Cassotta, Manuela; Armas Diaz, Yasmany; Cianciosi, Danila; Yang, Bei; Qi, Zexiu; Chen, Ge; Gracia Villar, Santos; Dzul López, Luis Alonso; Grosso, Giuseppe; Quiles, José L.; Xiao, Jianbo; Battino, Maurizio y Giampieri, Francesca mail manucassotta@gmail.com, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, santos.gracia@uneatlantico.es, luis.dzul@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es (2025) Single-cell omics for nutrition research: an emerging opportunity for human-centric investigations. Critical Reviews in Food Science and Nutrition. pp. 1-15. ISSN 1040-8398

Artículo Materias > Biomedicina
Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés This systematic review included 31 clinical trial articles examining the effects of natural compounds on Alzheimer’s disease (AD) and mild cognitive impairment (MCI), involving 3582 participants aged 50–90. Treatment durations ranged from 8 weeks to 2 years, with an average of 12.5 months. Notably, 11 studies focused on herbal extracts highlighting their prominence in current research. These extracts showed potential cognitive and neuroprotective benefits, although results varied across compounds and study designs. Other natural compounds—including flavonoids, polyphenols, omega-3 fatty acids, Aloe vera, Spirulina, and citrus phytochemicals—may provide cognitive and neuroprotective benefits, with ginseng and Ginkgo biloba combinations also showing promise. Curcumin and Melissa officinalis had limited effects, resveratrol showed mixed outcomes with some side effects, and matcha green tea may improve cognition and sleep quality. Despite generally favorable results, the studies varied considerably in design and quality; nonetheless, herbal extracts represent a prominent category of natural interventions in AD and MCI, underscoring the need for further large-scale, high-quality clinical trials to confirm their therapeutic potential. metadata Bayo Jimenez, Maria T.; Rivas-García, Lorenzo; Sánchez-González, Cristina; Grosso, Giuseppe; Lipari, Vivian; Vera-Ramírez, Laura; Battino, Maurizio; Giampieri, Francesca; Quiles, José L. y Forbes-Hernández, Tamara Y. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, vivian.lipari@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es, jose.quiles@uneatlantico.es, SIN ESPECIFICAR (2025) Natural Products in Alzheimer’s Disease: A Systematic Review of Clinical Trials and Underlying Molecular Mechanisms. International Journal of Molecular Sciences, 26 (21). p. 10631. ISSN 1422-0067

Artículo Materias > Biomedicina
Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica
Universidad Internacional do Cuanza > Investigación > Producción Científica
Universidad de La Romana > Investigación > Producción Científica Abierto Inglés Polyphenols are naturally occurring compounds that can be found in plant-based foods, including fruits, vegetables, nuts, seeds, herbs, spices, and beverages, the use of which has been linked to enhanced brain health and cognitive function. These natural molecules are broadly classified into two main groups: flavonoids and non-flavonoid polyphenols, the latter including phenolic acids, stilbenes, and tannins. Flavonoids are primarily known for their potent antioxidant properties, which help neutralize harmful reactive oxygen species (ROS) in the brain, thereby reducing oxidative stress, a key contributor to neurodegenerative diseases. In addition to their antioxidant effects, flavonoids have been shown to modulate inflammation, enhance neuronal survival, and support neurogenesis, all of which are critical for maintaining cognitive function. Phenolic acids possess strong antioxidant properties and are believed to protect brain cells from oxidative damage. Neuroprotective effects of these molecules can also depend on their ability to modulate signaling pathways associated with inflammation and neuronal apoptosis. Among polyphenols, hydroxycinnamic acids such as caffeic acid have been shown to enhance blood-brain barrier permeability, which may increase the delivery of other protective compounds to the brain. Another compound of interest is represented by resveratrol, a stilbene extensively studied for its potential neuroprotective properties related to its ability to activate the sirtuin pathway, a molecular signaling pathway involved in cellular stress response and aging. Lignans, on the other hand, have shown promise in reducing neuroinflammation and oxidative stress, which could help slow the progression of neurodegenerative diseases and cognitive decline. Polyphenols belonging to different subclasses, such as flavonoids, phenolic acids, stilbenes, and lignans, exert neuroprotective effects by regulating microglial activation, suppressing pro-inflammatory cytokines, and mitigating oxidative stress. These compounds act through multiple signaling pathways, including NF-κB, MAPK, and Nrf2, and they may also influence genetic regulation of inflammation and immune responses at brain level. Despite their potential for brain health and cognitive function, polyphenols are often characterized by low bioavailability, something that deserves attention when considering their therapeutic potential. Future translational studies are needed to better understand the right dosage, the overall diet, the correct target population, as well as ideal formulations allowing to overcome bioavailability limitations. metadata Godos, Justyna; Carota, Giuseppe; Caruso, Giuseppe; Micek, Agnieszka; Frias-Toral, Evelyn; Giampieri, Francesca; Brito Ballester, Julién; Battino, Maurizio; Rodríguez Velasco, Carmen Lilí y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, julien.brito@uneatlantico.es, maurizio.battino@uneatlantico.es, carmen.rodriguez@uneatlantico.es, jose.quiles@uneatlantico.es (2025) Molecular mechanisms underlying the neuroprotective effects of polyphenols: implications for cognitive function. EXCLI journal, 24. pp. 1262-1294. ISSN 1611-2156

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Garlic is a horticultural product highly valued for its culinary and medicinal attributes. The aim of this study was to evaluate the composition of a garlic hydrophilic extract as well as the influence on redox biology, Alzheimer's Disease (AD) markers and aging, using Caenorhabditis elegans as experimental model. The extract was rich in sulfur compounds, highlighting the presence of other compounds like phenolics, and the antioxidant property was corroborated. Regarding AD markers, the acetylcholinesterase inhibitory capacity was demonstrated in vitro. Although the extract did not modify the amyloid β-induced paralysis degree, it was able to improve, in a dose-dependent manner, some locomotive parameters affected by the hyperphosphorylated tau protein in C. elegans. It could be related to the effect found on GFP-transgenic stains, mainly regarding to the increase in the gene expression of HSP-16.2. Moreover, an initial investigation into the aging process revealed that the extract successfully inhibited the accumulation of intracellular and mitochondrial reactive oxygen species in aged worms. These results provide valuable insights into the multifaceted impact of garlic extract, particularly in the context of aging and neurodegenerative processes. This study lays a foundation for further research avenues exploring the intricate molecular mechanisms underlying garlic effects and its translation into potential therapeutic interventions for age-related neurodegenerative conditions. metadata Navarro‐Hortal, María D.; Romero‐Marquez, Jose M.; Ansary, Johura; Montalbán‐Hernández, Cristina; Varela‐López, Alfonso; Giampieri, Francesca; Xiao, Jianbo; Calderón Iglesias, Rubén; Battino, Maurizio; Sánchez‐González, Cristina; Forbes‐Hernández, Tamara Y. y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es (2025) Effects of a Garlic Hydrophilic Extract Rich in Sulfur Compounds on Redox Biology and Alzheimer's Disease Markers in Caenorhabditis Elegans. eFood, 6 (2). ISSN 2666-3066

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Alzheimer's disease (AD) involves β-amyloid plaques and tau hyperphosphorylation, driven by oxidative stress and neuroinflammation. Cyclooxygenase-2 (COX-2) and acetylcholinesterase (AChE) activities exacerbate AD pathology. Olive leaf (OL) extracts, rich in bioactive compounds, offer potential therapeutic benefits. This study aimed to assess the anti-inflammatory, anti-cholinergic, and antioxidant effects of three OL extracts (low, mid, and high bioactive content) in vitro and their protective effects against AD-related proteinopathies in Caenorhabditis elegans models. OL extracts were characterized for phenolic composition, AChE and COX-2 inhibition, as well as antioxidant capacity. Their effects on intracellular and mitochondrial reactive oxygen species (ROS) were tested in C. elegans models expressing human Aβ and tau proteins. Gene expression analyses examined transcription factors (DAF-16, skinhead [SKN]-1) and their targets (superoxide dismutase [SOD]-2, SOD-3, GST-4, and heat shock protein [HSP]-16.2). High-OL extract demonstrated superior AChE and COX-2 inhibition and antioxidant capacity. Low- and high-OL extracts reduced Aβ aggregation, ROS levels, and proteotoxicity via SKN-1/NRF-2 and DAF-16/FOXO pathways, whereas mid-OL showed moderate effects through proteostasis modulation. In tau models, low- and high-OL extracts mitigated mitochondrial ROS levels via SOD-2 but had limited effects on intracellular ROS levels. High-OL extract also increased GST-4 levels, whereas low and mid extracts enhanced GST-4 levels. OL extracts protect against AD-related proteinopathies by modulating oxidative stress, inflammation, and proteostasis. High-OL extract showed the most promise for nutraceutical development due to its robust phenolic profile and activation of key antioxidant pathways. Further research is needed to confirm long-term efficacy. metadata Romero‐Marquez, Jose M.; Navarro‐Hortal, María D.; Varela‐López, Alfonso; Calderón Iglesias, Rubén; Puentes, Juan G.; Giampieri, Francesca; Battino, Maurizio; Sánchez‐González, Cristina; Xiao, Jianbo; García‐Ruiz, Roberto; Sánchez, Sebastián; Forbes‐Hernández, Tamara Y. y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es (2025) Olive Leaf Extracts With High, Medium, or Low Bioactive Compounds Content Differentially Modulate Alzheimer's Disease via Redox Biology. Food Frontiers. ISSN 2643-8429

2024

Artículo Materias > Biomedicina
Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica
Universidad Internacional do Cuanza > Investigación > Producción Científica
Universidad de La Romana > Investigación > Producción Científica Abierto Inglés Cardiovascular diseases (CVDs) are one of the main causes of mortality and morbidity worldwide. A healthy diet rich in plant-derived compounds such as (poly)phenols appears to have a key role in improving cardiovascular health. Flavan-3-ols represent a subclass of (poly)phenols of great interest for their possible health benefits. In this review, we summarized the results of clinical studies on vascular outcomes of flavan-3-ol supplementation and we focused on the role of the microbiota in CVD. Clinical trials included in this review showed that supplementation with flavan-3-ols mostly derived from cocoa products significantly reduces blood pressure and improves endothelial function. Studies on catechins from green tea demonstrated better results when involving healthy individuals. From a mechanistic point of view, emerging evidence suggests that microbial metabolites may play a role in the observed effects. Their function extends beyond the previous belief of ROS scavenging activity and encompasses a direct impact on gene expression and protein function. Although flavan-3-ols appear to have effects on cardiovascular health, further studies are needed to clarify and confirm these potential benefits and the rising evidence of the potential involvement of the microbiota. metadata Godos, Justyna; Romano, Giovanni Luca; Laudani, Samuele; Gozzo, Lucia; Guerrera, Ida; Dominguez Azpíroz, Irma; Martínez Díaz, Raquel; Quiles, José L.; Battino, Maurizio; Drago, Filippo; Giampieri, Francesca; Galvano, Fabio y Grosso, Giuseppe mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, irma.dominguez@unini.edu.mx, raquel.martinez@uneatlantico.es, jose.quiles@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR (2024) Flavan-3-ols and Vascular Health: Clinical Evidence and Mechanisms of Action. Nutrients, 16 (15). p. 2471. ISSN 2072-6643

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Broccoli has gained popularity as a highly consumed vegetable due to its nutritional and health properties. This study aimed to evaluate the composition profile and the antioxidant capacity of a hydrophilic extract derived from broccoli byproducts, as well as its influence on redox biology, Alzheimer’s disease markers, and aging in the Caenorhabditis elegans model. The presence of glucosinolate was observed and antioxidant capacity was demonstrated both in vitro and in vivo. The in vitro acetylcholinesterase inhibitory capacity was quantified, and the treatment ameliorated the amyloid-β- and tau-induced proteotoxicity in transgenic strains via SOD-3 and SKN-1, respectively, and HSP-16.2 for both parameters. Furthermore, a preliminary study on aging indicated that the extract effectively reduced reactive oxygen species levels in aged worms and extended their lifespan. Utilizing broccoli byproducts for nutraceutical or functional foods could manage vegetable processing waste, enhancing productivity and sustainability while providing significant health benefits. metadata Navarro-Hortal, María D.; Romero-Márquez, Jose M.; López-Bascón, M. Asunción; Sánchez-González, Cristina; Xiao, Jianbo; Sumalla Cano, Sandra; Battino, Maurizio; Forbes-Hernande, Tamara Y. y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, sandra.sumalla@uneatlantico.es, maurizio.battino@uneatlantico.es, tamara.forbes@unini.edu.mx, jose.quiles@uneatlantico.es (2024) In Vitro and In Vivo Insights into a Broccoli Byproduct as a Healthy Ingredient for the Management of Alzheimer’s Disease and Aging through Redox Biology. Journal of Agricultural and Food Chemistry, 72 (10). pp. 5197-5211. ISSN 0021-8561

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Fundación Universitaria Internacional de Colombia > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional do Cuanza > Investigación > Producción Científica
Universidad de La Romana > Investigación > Producción Científica Abierto Inglés Cardiovascular diseases are among the leading causes of mortality worldwide, with dietary factors being the main risk contributors. Diets rich in bioactive compounds, such as (poly)phenols, have been shown to potentially exert positive effects on vascular health. Among them, resveratrol has gained particular attention due to its potential antioxidant and anti-inflammatory action. Nevertheless, the results in humans are conflicting possibly due to interindividual different responses. The gut microbiota, a complex microbial community that inhabits the gastrointestinal tract, has been called out as potentially responsible for modulating the biological activities of phenolic metabolites in humans. The present review aims to summarize the main findings from clinical trials on the effects of resveratrol interventions on endothelial and vascular outcomes and review potential mechanisms interesting the role of gut microbiota on the metabolism of this molecule and its cardioprotective metabolites. The findings from randomized controlled trials show contrasting results on the effects of resveratrol supplementation and vascular biomarkers without dose-dependent effect. In particular, studies in which resveratrol was integrated using food sources, i.e., red wine, reported significant effects although the resveratrol content was, on average, much lower compared to tablet supplementation, while other studies with often extreme resveratrol supplementation resulted in null findings. The results from experimental studies suggest that resveratrol exerts cardioprotective effects through the modulation of various antioxidant, anti-inflammatory, and anti-hypertensive pathways, and microbiota composition. Recent studies on resveratrol-derived metabolites, such as piceatannol, have demonstrated its effects on biomarkers of vascular health. Moreover, resveratrol itself has been shown to improve the gut microbiota composition toward an anti-inflammatory profile. Considering the contrasting findings from clinical studies, future research exploring the bidirectional link between resveratrol metabolism and gut microbiota as well as the mediating effect of gut microbiota in resveratrol effect on cardiovascular health is warranted. metadata Godos, Justyna; Romano, Giovanni Luca; Gozzo, Lucia; Laudani, Samuele; Paladino, Nadia; Dominguez Azpíroz, Irma; Martínez López, Nohora Milena; Giampieri, Francesca; Quiles, José L.; Battino, Maurizio; Galvano, Fabio; Drago, Filippo y Grosso, Giuseppe mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, irma.dominguez@unini.edu.mx, nohora.martinez@uneatlantico.es, francesca.giampieri@uneatlantico.es, jose.quiles@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2024) Resveratrol and vascular health: evidence from clinical studies and mechanisms of actions related to its metabolites produced by gut microbiota. Frontiers in Pharmacology, 15. ISSN 1663-9812

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica Abierto Inglés Over the last decades, the Mediterranean diet gained enormous scientific, social, and commercial attention due to proven positive effects on health and undeniable taste that facilitated a widespread popularity. Researchers have investigated the role of Mediterranean-type dietary patterns on human health all around the world, reporting consistent findings concerning its benefits. However, what does truly define the Mediterranean diet? The myriad of dietary scores synthesizes the nutritional content of a Mediterranean-type diet, but a variety of aspects are generally unexplored when studying the adherence to this dietary pattern. Among dietary factors, the main characteristics of the Mediterranean diet, such as consumption of fruit and vegetables, olive oil, and cereals should be accompanied by other underrated features, such as the following: (i) specific reference to whole-grain consumption; (ii) considering the consumption of legumes, nuts, seeds, herbs and spices often untested when exploring the adherence to the Mediterranean diet; (iii) consumption of eggs and dairy products as common foods consumed in the Mediterranean region (irrespectively of the modern demonization of dietary fat intake). Another main feature of the Mediterranean diet includes (red) wine consumption, but more general patterns of alcohol intake are generally unmeasured, lacking specificity concerning the drinking occasion and intensity (i.e., alcohol drinking during meals). Among other underrated aspects, cooking methods are rather simple and yet extremely varied. Several underrated aspects are related to the quality of food consumed when the Mediterranean diet was first investigated: foods are locally produced, minimally processed, and preserved with more natural methods (i.e., fermentation), strongly connected with the territory with limited and controlled impact on the environment. Dietary habits are also associated with lifestyle behaviors, such as sleeping patterns, and social and cultural values, favoring commensality and frugality. In conclusion, it is rather reductive to consider the Mediterranean diet as just a pattern of food groups to be consumed decontextualized from the social and geographical background of Mediterranean culture. While the methodologies to study the Mediterranean diet have demonstrated to be useful up to date, a more holistic approach should be considered in future studies by considering the aforementioned underrated features and values to be potentially applied globally through the concept of a “Planeterranean” diet. metadata Godos, Justyna; Scazzina, Francesca; Paternò Castello, Corrado; Giampieri, Francesca; Quiles, José L.; Briones Urbano, Mercedes; Battino, Maurizio; Galvano, Fabio; Iacoviello, Licia; de Gaetano, Giovanni; Bonaccio, Marialaura y Grosso, Giuseppe mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, jose.quiles@uneatlantico.es, mercedes.briones@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2024) Underrated aspects of a true Mediterranean diet: understanding traditional features for worldwide application of a “Planeterranean” diet. Journal of Translational Medicine, 22 (1). ISSN 1479-5876

Artículo Materias > Biomedicina
Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica
Universidad Internacional do Cuanza > Investigación > Producción Científica
Universidad de La Romana > Investigación > Producción Científica Abierto Inglés Isoflavones are a group of (poly)phenols, also defined as phytoestrogens, with chemical structures comparable with estrogen, that exert weak estrogenic effects. These phytochemical compounds have been targeted for their proven antioxidant and protective effects. Recognizing the increasing prevalence of cardiovascular diseases (CVD), there is a growing interest in understanding the potential cardiovascular benefits associated with these phytochemical compounds. Gut microbiota may play a key role in mediating the effects of isoflavones on vascular and endothelial functions, as it is directly implicated in isoflavones metabolism. The findings from randomized clinical trials indicate that isoflavone supplementation may exert putative effects on vascular biomarkers among healthy individuals, but not among patients affected by cardiometabolic disorders. These results might be explained by the enzymatic transformation to which isoflavones are subjected by the gut microbiota, suggesting that a diverse composition of the microbiota may determine the diverse bioavailability of these compounds. Specifically, the conversion of isoflavones in equol—a microbiota-derived metabolite—seems to differ between individuals. Further studies are needed to clarify the intricate molecular mechanisms behind these contrasting results. metadata Laudani, Samuele; Godos, Justyna; Romano, Giovanni Luca; Gozzo, Lucia; Di Domenico, Federica Martina; Dominguez Azpíroz, Irma; Martínez Díaz, Raquel; Giampieri, Francesca; Quiles, José L.; Battino, Maurizio; Drago, Filippo; Galvano, Fabio y Grosso, Giuseppe mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, irma.dominguez@unini.edu.mx, raquel.martinez@uneatlantico.es, francesca.giampieri@uneatlantico.es, jose.quiles@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2024) Isoflavones Effects on Vascular and Endothelial Outcomes: How Is the Gut Microbiota Involved? Pharmaceuticals, 17 (2). p. 236. ISSN 1424-8247

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Cerrado Inglés In this study, the phytochemical profile of fifty olive leaves (OL) extracts from Spain, Italy, Greece, Portugal, and Morocco was characterized and their anti-cholinergic, anti-inflammatory, and antioxidant activities were evaluated. Luteolin-7-O-glucoside, isoharmnentin, and apigenin were involved in the acetylcholinesterase (AChE) inhibitory activity, while oleuropein and hydroxytyrosol showed noteworthy potential. Secoiridoids contributed to the cyclooxygenase-2 inhibitory activity and antioxidant capacity. Compounds such as oleuropein, ligstroside and luteolin-7-O-glucoside, may exert an important role in the ferric reducing antioxidant capacity. It should be also highlighted the role of hydroxytyrosol, hydroxycoumarins, and verbascoside concerning the antioxidant activity. This research provides valuable insights and confirms that specific compounds within OL extracts contribute to distinct anti-cholinergic, anti-inflammatory, and anti-oxidative effects. metadata Romero-Márquez, Jose M.; Navarro-Hortal, María D.; Forbes-Hernández, Tamara Y.; Varela-López, Alfonso; Puentes, Juan G.; Sánchez-González, Cristina; Sumalla Cano, Sandra; Battino, Maurizio; García-Ruiz, Roberto; Sánchez, Sebastián y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, sandra.sumalla@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es (2024) Effect of olive leaf phytochemicals on the anti-acetylcholinesterase, anti-cyclooxygenase-2 and ferric reducing antioxidant capacity. Food Chemistry, 444. p. 138516. ISSN 03088146

2023

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Fundación Universitaria Internacional de Colombia > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica Cerrado Inglés Manuka honey, which is rich in pinocembrin, quercetin, naringenin, salicylic, p-coumaric, ferulic, syringic and 3,4-dihydroxybenzoic acids, has been shown to have pleiotropic effects against colon cancer cells. In this study, potential chemosensitizing effects of Manuka honey against 5-Fluorouracil were investigated in colonspheres enriched with cancer stem cells (CSCs), which are responsible for chemoresistance. Results showed that 5-Fluorouracil increased when it was combined with Manuka honey by downregulating the gene expression of both ATP-binding cassette sub-family G member 2, an efflux pump and thymidylate synthase, the main target of 5-Fluorouracil which regulates the ex novo DNA synthesis. Manuka honey was associated with decreased self-renewal ability by CSCs, regulating expression of several genes in Wnt/β-catenin, Hedgehog and Notch pathways. This preliminary study opens new areas of research into the effects of natural compounds in combination with pharmaceuticals and, potentially, increase efficacy or reduce adverse effects. metadata Cianciosi, Danila; Armas Diaz, Yasmany; Alvarez-Suarez, José M.; Chen, Xiumin; Zhang, Di; Martínez López, Nohora Milena; Briones Urbano, Mercedes; Quiles, José L.; Amici, Adolfo; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, nohora.martinez@uneatlantico.es, mercedes.briones@uneatlantico.es, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es (2023) Can the phenolic compounds of Manuka honey chemosensitize colon cancer stem cells? A deep insight into the effect on chemoresistance and self-renewal. Food Chemistry, 427. p. 136684. ISSN 03088146

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Fundación Universitaria Internacional de Colombia > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica Cerrado Inglés Diets enriched in plant-based foods are associated with the maintenance of a good well-being and with the prevention of many non-communicable diseases. The health effects of fruits and vegetables consumption are mainly due to the presence of micronutrients, including vitamins and minerals, and polyphenols, plant secondary metabolites. One of the most important classes of phenolic compounds are anthocyanins, that confer the typical purple-red color to many foods, such as berries, peaches, plums, red onions, purple corn, eggplants, as well as purple carrots, sweet potatoes and red cabbages, among others. This commentary aims to briefly highlight the progress made by science in the last years, focusing on some unexpected aspects related with anthocyanins, such as their bioavailability, their health effects and their relationship with gut microbiota metadata Giampieri, Francesca; Cianciosi, Danila; Alvarez-Suarez, José M.; Quiles, José L.; Forbes-Hernández, Tamara Y.; Navarro-Hortal, María D.; Machì, Michele; Pali-Casanova, Ramón; Martínez Espinosa, Julio César; Chen, Xiumin; Zhang, Di; Bai, Weibin; Lingmin, Tian; Mezzetti, Bruno; Battino, Maurizio y Diaz, Yasmany Armas mail francesca.giampieri@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, ramon.pali@unini.edu.mx, ulio.martinez@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR (2023) Anthocyanins: what do we know until now? Journal of Berry Research. pp. 1-6. ISSN 18785093

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional do Cuanza > Investigación > Producción Científica Abierto Inglés Alzheimer’s Disease (AD) is the cause of around 60–70% of global cases of dementia and approximately 50 million people have been reported to suffer this disease worldwide. The leaves of olive trees (Olea europaea) are the most abundant by-products of the olive grove industry. These by-products have been highlighted due to the wide variety of bioactive compounds such as oleuropein (OLE) and hydroxytyrosol (HT) with demonstrated medicinal properties to fight AD. In particular, the olive leaf (OL), OLE, and HT reduced not only amyloid-β formation but also neurofibrillary tangles formation through amyloid protein precursor processing modulation. Although the isolated olive phytochemicals exerted lower cholinesterase inhibitory activity, OL demonstrated high inhibitory activity in the cholinergic tests evaluated. The mechanisms underlying these protective effects may be associated with decreased neuroinflammation and oxidative stress via NF-κB and Nrf2 modulation, respectively. Despite the limited research, evidence indicates that OL consumption promotes autophagy and restores loss of proteostasis, which was reflected in lower toxic protein aggregation in AD models. Therefore, olive phytochemicals may be a promising tool as an adjuvant in the treatment of AD. metadata Romero-Márquez, Jose M.; Forbes-Hernández, Tamara Y.; Navarro-Hortal, María D.; Quirantes-Piné, Rosa; Grosso, Giuseppe; Giampieri, Francesca; Lipari, Vivian; Sánchez-González, Cristina; Battino, Maurizio y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, vivian.lipari@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, jose.quiles@uneatlantico.es (2023) Molecular Mechanisms of the Protective Effects of Olive Leaf Polyphenols against Alzheimer’s Disease. International Journal of Molecular Sciences, 24 (5). p. 4353. ISSN 1422-0067

2022

Artículo Materias > Biomedicina
Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Alzheimer's is a chronic degenerative disease of the central nervous system considered the leading cause of dementia in the world. It is characterized by two etiopathological events related to oxidative stress: the aggregation of β-amyloid peptide and the formation of neurofibrillary tangles of hyperphosphorylated Tau protein in the brain. The incidence of this disease increases with age and has been associated with inadequate lifestyles. Some natural compounds have been shown to improve the hallmarks of the disease. However, despite its potential, there is no scientific evidence about Manuka honey (MH) in this regard. In the present work we evaluated the effect of MH on the toxicity induced by Aβ aggregation and Tau in a Caenorhabditis elegans model. Our results demonstrated that MH was able to improve indicators of oxidative stress and delayed Aβ-induced paralysis in the AD model CL4176 through HSP-16.2 and SKN-1/NRF2 pathways. Nevertheless, its sugar content impaired the indicators of locomotion (an indicator of tau neurotoxicity) in both the transgenic strain BR5706 and in the wild-type N2 worms. metadata Navarro-Hortal, María D.; Romero-Márquez, Jose M.; Muñoz-Ollero, Pedro; Jiménez-Trigo, Victoria; Esteban-Muñoz, Adelaida; Tutusaus, Kilian; Giampieri, Francesca; Battino, Maurizio; Sánchez-González, Cristina; Rivas-García, Lorenzo; Llopis, Juan; Forbes-Hernández, Tamara Y. y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, kilian.tutusaus@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es (2022) Amyloid β-but not Tau-induced neurotoxicity is suppressed by Manuka honey via HSP-16.2 and SKN-1/Nrf2 pathways in an in vivo model of Alzheimer's disease. Food & Function. ISSN 2042-6496

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional do Cuanza > Investigación > Producción Científica Abierto Inglés Age-related macular degeneration (AMD) is a serious degenerative disease affecting the eyes, and is the main cause of severe vision loss among people >55 years of age in developed countries. Its onset and progression have been associated with several genetic and lifestyle factors, with diet appearing to play a pivotal role in the latter. In particular, dietary eating patterns rich in plant foods have been shown to lower the risk of developing the disease, and to decrease the odds of progressing to more advanced stages in individuals already burdened with early AMD. We systematically reviewed the literature to analyse the relationship between the adherence to a Mediterranean diet, a mainly plant-based dietary pattern, and the onset/progression of AMD. Eight human observational studies were analysed. Despite some differences, they consistently indicate that higher adherence to a Mediterranean eating pattern lowers the odds of developing AMD and decreases the risk of progression to more advanced stages of the disease, establishing the way for preventative measures emphasizing dietary patterns rich in plant-foods metadata Gastaldello, Annalisa; Giampieri, Francesca; Quiles, José L.; Navarro-Hortal, María D.; Aparicio Obregón, Silvia; García Villena, Eduardo; Tutusaus, Kilian; De Giuseppe, Rachele; Grosso, Giuseppe; Cianciosi, Danila; Forbes-Hernández, Tamara Y.; Nabavi, Seyed M. y Battino, Maurizio mail SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, silvia.aparicio@uneatlantico.es, eduardo.garcia@uneatlantico.es, kilian.tutusaus@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2022) Adherence to the Mediterranean-Style Eating Pattern and Macular Degeneration: A Systematic Review of Observational Studies. Nutrients, 14 (10). p. 2028. ISSN 2072-6643

2021

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros
Universidad Internacional do Cuanza > Investigación > Producción Científica Cerrado Inglés Fasting, caloric restriction and foods or compounds mimicking the biological effects of caloric restriction, known as caloric restriction mimetics, have been associated with a lower risk of age-related diseases, including cardiovascular diseases, cancer and cognitive decline, and a longer lifespan. Reduced calorie intake has been shown to stimulate cancer immunosurveillance, reducing the migration of immunosuppressive regulatory T cells towards the tumor bulk. Autophagy stimulation via reduction of lysine acetylation, increased sensitivity to chemo- and immunotherapy, along with a reduction of insulin-like growth factor 1 and reactive oxygen species have been described as some of the major effects triggered by caloric restriction. Fasting and caloric restriction have also been shown to beneficially influence gut microbiota composition, modify host metabolism, reduce total cholesterol and triglyceride levels, lower diastolic blood pressure and elevate morning cortisol level, with beneficial modulatory effects on cardiopulmonary fitness, body fat and weight, fatigue and weakness, and general quality of life. Moreover, caloric restriction may reduce the carcinogenic and metastatic potential of cancer stem cells, which are generally considered responsible of tumor formation and relapse. Here, we reviewed in vitro and in vivo studies describing the effects of fasting, caloric restriction and some caloric restriction mimetics on immunosurveillance, gut microbiota, metabolism, and cancer stem cell growth, highlighting the molecular and cellular mechanisms underlying these effects. Additionally, studies on caloric restriction interventions in cancer patients or cancer risk subjects are discussed. Considering the promising effects associated with caloric restriction and caloric restriction mimetics, we think that controlled-randomized large clinical trials are warranted to evaluate the inclusion of these non-pharmacological approaches in clinical practice. metadata Pistollato, Francesca; Forbes-Hernández, Tamara Y.; Calderón Iglesias, Rubén; Ruiz Salces, Roberto; Elexpuru Zabaleta, Maria; Dominguez Azpíroz, Irma; Cianciosi, Danila; Quiles, José L.; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, maria.elexpuru@uneatlantico.es, irma.dominguez@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2021) Effects of caloric restriction on immunosurveillance, microbiota and cancer cell phenotype: Possible implications for cancer treatment. Seminars in Cancer Biology. pp. 45-57. ISSN 1044-579X

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Age-related bone disorders such as osteoporosis or osteoarthritis are a major public health problem due to the functional disability for millions of people worldwide. Furthermore, fractures are associated with a higher degree of morbidity and mortality in the long term, which generates greater financial and health costs. As the world population becomes older, the incidence of this type of disease increases and this effect seems notably greater in those countries that present a more westernized lifestyle. Thus, increased efforts are directed toward reducing risks that need to focus not only on the prevention of bone diseases, but also on the treatment of persons already afflicted. Evidence is accumulating that dietary lipids play an important role in bone health which results relevant to develop effective interventions for prevent bone diseases or alterations, especially in the elderly segment of the population. This review focuses on evidence about the effects of dietary lipids on bone health and describes possible mechanisms to explain how lipids act on bone metabolism during aging. Little work, however, has been accomplished in humans, so this is a challenge for future research. metadata Romero-Márquez, Jose M.; Varela-López, Alfonso; Navarro-Hortal, María D.; Badillo-Carrasco, Alberto; Forbes-Hernández, Tamara Y.; Giampieri, Francesca; Dominguez Azpíroz, Irma; Madrigal-Hoyos, Lorena; Battino, Maurizio y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, irma.dominguez@uneatlantico.es, lorena.madrigal@uneatlantico.es, maurizio.battino@uneatlantico.es, jose.quiles@uneatlantico.es (2021) Molecular Interactions between Dietary Lipids and Bone Tissue during Aging. International Journal of Molecular Sciences, 22 (12). p. 6473. ISSN 1422-0067

Artículo Materias > Biomedicina Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Low energy pulsed electromagnetic signals (PEMS) therapy, in the field of bioelectronics, has been suggested as a promising analgesic therapy with special interest in treating conditions with poor response to pharmacotherapy. This study evaluated the effectiveness of PEMS therapy on the treatment of chronic low back pain patients with a neuropathic component. A group of 64 individuals with such condition was allocated to a 2-week treatment period (10 twenty-minute sessions on consecutive days) with an active PEMS therapy device or an inactive device in random order. The pain was assessed on a visual analog scale, and the functional status was assessed using the SF-12 questionnaire. The visual analog scale scores were lower after treatment than at baseline but only in the group treated with the active device. According to the DN4 score, neuropathic pain decreased in both experimental groups with respect to baseline, but this was only significant for the group treated with the active device. Similarly, an improvement in the SF-12 and Medical Outcomes Study (MOS) sleep scale components was reported. The study demonstrated that low-energy PEMS therapy was efficient in reducing pain and improving function in chronic low back pain patients with a neuropathic component. metadata de Teresa, Carlos; Varela-López, Alfonso; Rios-Álvarez, Susana; Gálvez, Rafael; Maire, Coralie; Gracia Villar, Santos; Battino, Maurizio y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, santos.gracia@uneatlantico.es, maurizio.battino@uneatlantico.es, jose.quiles@uneatlantico.es (2021) Evaluation of the Analgesic Efficacy of a Bioelectronic Device in Non-Specific Chronic Low Back Pain with Neuropathic Component. A Randomized Trial. Journal of Clinical Medicine, 10 (8). p. 1781. ISSN 2077-0383

2018

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Cerrado Inglés During the process of beeswax recycling, many industrial derivatives are obtained. These matrices may have an interesting healthy and commercial potential but to date they have not been properly studied. The aim of the present work was to evaluate the proximal and phytochemical composition, the antioxidant capacity and cytotoxic effects of two by-products from beeswax recycling process named MUD 1 and MUD 2 on liver hepatocellular carcinoma. Our results showed that MUD 1 presented the highest (P < .05) fiber, protein, carbohydrate, polyphenol and flavonoid concentration, as well as the highest (P < .05) total antioxidant capacity than the MUD 2 samples. MUD1 exerted also anticancer activity on HepG2 cells, by reducing cellular viability, increasing intracellular ROS levels and affecting mitochondrial functionality in a dose-dependent manner. We showed for the first time that by-products from beeswax recycling process can represent a rich source of phytochemicals with high total antioxidant capacity and anticancer activity; however, further researches are necessary to evaluate their potentiality for human health by in vivo studies. metadata Giampieri, Francesca; Quiles, José L.; Orantes-Bermejo, Francisco J.; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Sánchez-González, Cristina; Llopis, Juan; Rivas-García, Lorenzo; Afrin, Sadia; Varela-López, Alfonso; Cianciosi, Danila; Reboredo-Rodriguez, Patricia; Fernández-Piñar, Cristina Torres; Caderón Iglesia, Rubén; Ruiz Salces, Roberto; Aparicio Obregón, Silvia; Crespo-Álvarez, Jorge; Dzul Lopez, Luis; Xiao, Jianbo y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, silvia.aparicio@uneatlantico.es, jorge.crespo@uneatlantico.es, luis.dzul@unini.edu.mx, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Are by-products from beeswax recycling process a new promising source of bioactive compounds with biomedical properties? Food and Chemical Toxicology, 112. pp. 126-133. ISSN 0278-6915

2017

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Regulation of lipid metabolism is essential for treatment and prevention of several chronic diseases such as obesity, diabetes, and cardiovascular diseases, which are responsible for most deaths worldwide. It has been demonstrated that the AMP-activated protein kinase (AMPK) has a direct impact on lipid metabolism by modulating several downstream-signaling components. The main objective of the present work was to evaluate the in vitro effect of a methanolic strawberry extract on AMPK and its possible repercussion on lipid metabolism in human hepatocellular carcinoma cells (HepG2). For such purpose, the lipid profile and the expression of proteins metabolically related to AMPK were determined on cells lysates. The results demonstrated that strawberry methanolic extract decreased total cholesterol, low-density lipoprotein (LDL)-cholesterol, and triglycerides levels (up to 0.50-, 0.30-, and 0.40-fold, respectively) while it stimulated the p-AMPK/AMPK expression (up to 3.06-fold), compared to the control. AMPK stimulation led to the phosphorylation and consequent inactivation of acetyl coenzyme A carboxylase (ACC) and inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), the major regulators of fatty acids and cholesterol synthesis, respectively. Strawberry treatment also entailed a 4.34-, 2.37-, and 2.47-fold overexpression of LDL receptor, sirtuin 1 (Sirt1), and the peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α), respectively, compared to control. The observed results were counteracted by treatment with compound C, an AMPK pharmacological inhibitor, confirming that multiple effects of strawberries on lipid metabolism are mediated by the activation of this protein. metadata Forbes-Hernandez, Tamara Y.; Giampieri, Francesca; Gasparrini, Massimiliano; Afrin, Sadia; Mazzoni, Luca; Cordero, Mario; Mezzetti, Bruno; Quiles, José L. y Battino, Maurizio mail tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation. Nutrients, 9 (6). p. 621. ISSN 2072-6643

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés A protracted pro-inflammatory state is a major contributing factor in the development, progression and complication of the most common chronic pathologies. Fruit and vegetables represent the main sources of dietary antioxidants and their consumption can be considered an efficient tool to counteract inflammatory states. In this context an evaluation of the protective effects of strawberry extracts on inflammatory stress induced by E. coli LPS on human dermal fibroblast cells was performed in terms of viability assays, ROS and nitrite production and biomarkers of oxidative damage of the main biological macromolecules. The results demonstrated that strawberry extracts exerted an anti-inflammatory effect on LPS-treated cells, through an increase in cell viability, and the reduction of ROS and nitrite levels, and lipid, protein and DNA damage. This work showed for the first time the potential health benefits of strawberry extract against inflammatory and oxidative stress in LPS-treated human dermal fibroblast cells. metadata Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Giampieri, Francesca; Afrin, Sadia; Mezzetti, Bruno; Quiles, José L.; Bompadre, Stefano y Battino, Maurizio mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Protective Effect of Strawberry Extract against Inflammatory Stress Induced in Human Dermal Fibroblasts. Molecules, 22 (1). p. 164. ISSN 1420-3049

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Dyslipidemia and oxidation of low density lipoproteins (LDL) are recognized as critical factors in the development of atherosclerosis. Healthy dietary patterns, with abundant fruit and vegetable consumption, may prevent the onset of these risk factors due to the presence of phytochemical compounds. Strawberries are known for their high content of polyphenols; among them, flavonoids are the major constituents, and it is presumed that they are responsible for the biological activity of the fruit. Nevertheless, there are only a few studies that actually evaluate the effects of different fractions isolated from strawberries. In order to assess the effects of two different strawberry extracts (whole methanolic extract/anthocyanin-enriched fraction) on the lipid profile and antioxidant status in human hepatocellular carcinoma (HepG2) cells, the triglycerides and LDL-cholesterol content, lipid peroxidation, intracellular reactive oxygen species (ROS) content and antioxidant enzymes’ activity on cell lysates were determined. Results demonstrated that both strawberry extracts not only improved the lipid metabolism by decreasing triglycerides and LDL-cholesterol contents, but also improved the redox state of HepG2 cells by modulating thiobarbituric acid-reactive substances production, antioxidant enzyme activity and ROS generation. The observed effects were more pronounced for the anthocyanin-enriched fraction. metadata Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Afrin, Sadia; Cianciosi, Danila; González-Paramás, Ana; Santos-Buelga, Celestino; Mezzetti, Bruno; Quiles, José L.; Battino, Maurizio; Giampieri, Francesca y Bompadre, Stefano mail tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR (2017) Strawberry (cv. Romina) Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells. International Journal of Molecular Sciences, 18 (6). p. 1149. ISSN 1422-0067

Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Abierto Inglés Extreme exposure of skin to Ultraviolet A (UVA)-radiation may induce a dysregulated production of reactive oxygen species (ROS) which can interact with cellular biomolecules leading to oxidative stress, inflammation, DNA damage, and alteration of cellular molecular pathways, responsible for skin photoaging, hyperplasia, erythema, and cancer. For these reasons, the use of dietary natural bioactive compounds with remarkable antioxidant activity could be a strategic tool to counteract these UVA-radiation-caused deleterious effects. Thus, the purpose of the present work was to test the efficacy of strawberry (50 μg/mL)-based formulations supplemented with Coenzyme Q10 (100 μg/mL) and sun protection factor 10 in human dermal fibroblasts irradiated with UVA-radiation. The apoptosis rate, the amount of intracellular reactive oxygen species (ROS) production, the expression of proteins involved in antioxidant and inflammatory response, and mitochondrial functionality were evaluated. The results showed that the synergic topical use of strawberry and Coenzyme Q10 provided a significant (p < 0.05) photoprotective effect, reducing cell death and ROS, increasing antioxidant defense, lowering inflammatory markers, and improving mitochondrial functionality. The obtained results suggest the use of strawberry-based formulations as an innovative, natural, and useful tool for the prevention of UVA exposure-induced skin diseases in order to decrease or substitute the amount of synthetic sunscreen agents. metadata Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Afrin, Sadia; Reboredo-Rodriguez, Patricia; Cianciosi, Danila; Mezzetti, Bruno; Quiles, José L.; Bompadre, Stefano; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR (2017) Strawberry-Based Cosmetic Formulations Protect Human Dermal Fibroblasts against UVA-Induced Damage. Nutrients, 9 (6). p. 605. ISSN 2072-6643

2015

Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica
Universidad Internacional Iberoamericana México > Investigación > Artículos y libros Cerrado Inglés Berries are a relevant source of micronutrients and nonessential phytochemicals, such as polyphenol compounds, that play a synergistic and cumulative role in human health promotion. Several systematic analyses showed that berry phenolics are able to detoxify reactive oxygen and nitrogen species, blocking their production, to intervene in the cell cycle, participating in the transduction and expression of genes involved in apoptosis, and to repair oxidative DNA damage. As a consequence, the improvement of the nutritional quality of berries has become a new quality target of breeding and biotechnological strategies, to control or to increase the content of specific health-related compounds in fruits. This work reviews, on the basis of the in vitro and in vivo evidence, the main berries' phytochemical compounds and their possible mechanisms of action on pathways involved in several type of diseases, with particular attention to cancer, inflammation, neurodegeneration, diabetes and cardiovascular diseases. © 2015 Society of Chemical Industry metadata Mazzoni, Luca; Perez-Lopez, Patricia; Giampieri, Francesca; Alvarez-Suarez, José M.; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Quiles, José L.; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2015) The genetic aspects of berries: from field to health. Journal of the Science of Food and Agriculture, 96 (2). pp. 365-371. ISSN 00225142